气相色谱测定固态酿造基质中四甲基吡嗪的前处理方法优化

1345,沈 23,郭 1345,陈 23,王超凯13456,王 西23,李国红1345,常少健1345,李 13456,张 13456*

1.中国轻工业酿酒工程及应用重点实验室,四川成都 611130

2.四川郎酒股份有限公司,四川古蔺 646523

3.四川省酱香型白酒生态酿造工程技术研究中心,四川古蔺 646523

4.四川省食品发酵工业研究设计院有限公司,四川成都 611130

5.刘念酿酒师技能大师工作室,四川成都 6111306.酿酒生物技术及应用四川省重点实验室,四川宜宾 644000

摘要:对气相色谱测定固态酿造基质中四甲基吡嗪的前处理方法进行了优化,水溶液浸提pH5,液液萃取的萃取剂为乙酸乙酯,萃取时间为15 min。高温大曲和堆积糟醅中四甲基吡嗪测定的精密度分别为2.71%9.59%,加标回收率分别在84.64%93.11%83.33%101.04%

关键词:四甲基吡嗪;前处理;固态酿造;气相色谱

中图分类号:TS207.3   文献标识码:A   文章编号:1674-506X202104-0127-0004


Optimization of the Pretreatment Method for the Determination of Tetramethylpyrazide in Solid Brewing Matrix by Gas Chromatography

PENG Kui1345, SHEN Yi23, GUO Jie1345, CHEN Bo23, WANG Chao-kai13456, WANG Xi23, LI Guo-hong1345, CHANG Shao-jian1345, LI Mi13456, ZHANG Lei13456*

1.Key Laboratory of Brewing Engineering and Application of China National Light Industry Council, Chengdu Sichuan 611130, China;

2.Sichuan Langjiu Co., Ltd., Gulin Sichuan 646523 China;

3.Sichuan Maotai-flavor Liquor Ecological Brewing Engineering Technology Research Center, Gulin Sichuan 646523, China;

4.Sichuan Food and Fermentation Industry Research & Design Institute Co., Ltd., Chengdu Sichuan 611130, China;

5. Liu Nian's Master Studio of Oenological Skills, Chengdu Sichuan 611130, China;

6.Liquor Making Bio-Technology & Application of Key Laboratory of Sichuan Province, Yibin Sichuan 644000, China

AbstractThe pretreatment method for the determination of tetramethylpyrazide (TTMP) in solid brewing matrix by gas chromatography was optimized. The pH of aqueous solution was 5,  the extractant of liquid-liquid extraction was ethyl acetate, and the extraction time was 15 minutes. The precision of determination of TTMP in high temperature Daqu and accumulated fermented grains was 2.71% and 9.59%, respectively. The recovery rate was 84.64%~93.11% and 83.33%~101.04%, respectively.

Keywordstetramethylpyrazide; pretreatment; solid brewing; gas chromatography

doi10.3969/j.issn.1674-506X.2021.04-019


收稿日期:2021-01-26

基金项目:四川省科技厅重点研发项目(2019YFS0004);四川省科技厅重点研发项目(2019YFN0006);四川省科技厅重点研发项目(2019YFS0521);酿酒生物技术及应用四川省重点实验室(NJ2019-03NJ2019-07

作者简介:彭奎(1982-),男,硕士,高级工程师。研究方向:酿酒工程。

*通信作者


引文格式:彭奎,沈毅,郭杰,.气相色谱测定固态酿造基质中四甲基吡嗪的前处理方法优化[J].食品与发酵科技,2021,57(4):127-130.


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